SB6.0: Where Biology Became Engineering and A New Science Was Born

The landmark conference that transformed synthetic biology from discovery science to engineering discipline

Synthetic Biology Bioengineering Minimal Genome SB6.0 Conference

Introduction: The Conference That Redefined Life's Blueprint

In July 2013, on the bustling campus of Imperial College London, something remarkable was unfolding. Over 700 scientists, engineers, ethicists, and entrepreneurs from around the globe had gathered for what would become a landmark moment in modern science: SB6.0, the 6th International Meeting on Synthetic Biology 1 .

Global Gathering

700+ participants from diverse scientific backgrounds united by a common vision of engineering biology.

Transformative Vision

Shifting biology from discovery science to engineering discipline with predictable, standardized approaches.

The SB6.0 Conference: A Global Converging Point

SB6.0 represented the pinnacle of a conference series organized by the BioBricks Foundation, designed specifically as "the preeminent academic meeting in synthetic biology" 1 . What made this gathering exceptional wasn't just its scale—though with over 700 attendees it was the largest synthetic biology conference to date—but its deliberate global character and interdisciplinary nature 1 .

Systematic Engineering Meets Biological Complexity

Exploring how engineering principles can be applied to complex biological systems

Connecting People and Technologies

Building collaborative networks across disciplines and institutions

Engineering Biology for New Materials

Developing novel biomaterials with customized properties

Conference Statistics
Attendees: 700+
Duration: 3 days
Location: Imperial College London
Organizer: BioBricks Foundation

What Is Synthetic Biology? The Science of Engineering Life

"Synthetic biology is the design and construction of new biological devices and systems—or the redesign of existing natural biological systems—for useful practical purposes" 2 .
DNA-based Device Construction

Creating genetic circuits that can perform logic operations, much like electronic circuits.

Genome-driven Cell Engineering

Reprogramming entire cellular systems by writing or extensively editing genomes.

Protocell Creation

Building simple, cell-like entities from non-living components to understand life's principles.

Approach Core Objective Example Applications
DNA-based Device Construction Create genetic circuits with predictable functions Biological sensors, cellular computers
Genome-driven Cell Engineering Reprogram entire organisms by designing their genomes Microbes that produce pharmaceuticals, minimized cells
Protocell Creation Build simple cell-like systems from non-living components Artificial cells for drug delivery, understanding life's origins

The "Drive to Make": How Creating Shapes Knowing

A recurring theme both at SB6.0 and throughout synthetic biology is what researchers call the "drive to make"—a fundamental commitment to creating and producing that shapes how the field defines itself, conducts research, and produces knowledge 7 .

Methodological Impact

Strong emphasis on standardization, modularity, and predictability—hallmarks of engineering disciplines. The development of BioBricks exemplifies this methodological commitment 1 6 .

Epistemological Shift

The field's drive to make influences what counts as valuable knowledge. Knowing-how often takes precedence over knowing-why, and successful construction serves as validation of understanding 9 .

Key Insight

"Synthetic biology delivers knowledge via synthesis" that would be difficult or impossible to attain through traditional analytical approaches alone 9 .

Engineering Life: The Quest for a Minimal Genome

One of the most ambitious goals discussed at SB6.0 was the quest to create a minimal bacterial cell—an organism stripped down to only the genes essential for life. This project represents synthetic biology's ultimate test: to not merely tweak existing life but to fundamentally redefine it through what's known as the "top-down" approach 6 .

Methodology: Step-by-Step Genome Reduction
  1. Selection of a Simple Host: Researchers begin with a bacterium that has a naturally small genome 6 .
  2. Gene Identification and Classification: Using computational tools to identify essential genes.
  3. Systematic Genome Reduction: Precisely removing non-essential genome regions.
  4. Functional Validation: Testing whether minimized organisms can still perform basic life functions.
  5. Iterative Refinement: Moving incrementally toward the absolute minimum genome.
Genome Reduction Progress
Bacterial Species Original Genome Size Minimized Genome Size Reduction Percentage
Mycoplasma mycoides ~1,100 genes ~500 genes ~55%
Mesoplasma florum ~700 genes ~400 genes ~43%
Escherichia coli ~4,300 genes ~3,000 genes ~30%

The Scientist's Toolkit: Essential Tools for Engineering Biology

The ambitious projects showcased at SB6.0 depend on an array of sophisticated tools and reagents. These technologies form the essential toolkit that makes synthetic biology possible.

Reagent/Material Primary Function Application Examples
DNA Polymerases Amplify DNA sequences PCR, DNA assembly
Restriction Enzymes Cut DNA at specific sequences Golden Gate assembly, cloning
Ligases Join DNA fragments together DNA circuit construction
BioBrick Parts Standardized genetic components Modular genetic circuit design
Competent Cells Host cells for DNA transformation Plasmid propagation, protein expression
Laboratory Equipment
  • PCR machines for DNA amplification
  • Gel electrophoresis systems
  • DNA synthesizers
  • Microplate readers
  • Fluorescence microscopes
Emerging Technologies
  • Advanced DNA synthesis technologies
  • Gene editing tools like CRISPR
  • Automated workflow systems
  • High-throughput screening platforms
  • Computational design software

The Legacy of SB6.0: From Conference to Global Impact

The reverberations from SB6.0 extended far beyond the conference halls of Imperial College. The research presented and connections forged during those three days in July 2013 continued to shape synthetic biology for years to come.

Special Issue

Conference highlights documented in ACS Synthetic Biology

Standardization

Advancement of standardized biological parts and methods

Global Applications

Solutions for health, energy, and sustainability challenges

Continuing Impact

The foundational work presented at SB6.0 has paved the way for engineered organisms that produce advanced biofuels, precision medicines, and novel biomaterials with properties not found in nature .

References